Rouhani was handed replicas of the Iran national football team No 12 jerseys and he wished the team well ahead of their 2018 FIFA World Cup Russia preparations.
“I would like to express my happiness to participate at Team Melli’s farewell ceremony. I want to thank you because you made Iranian people happy by booking a place in the 2018 World Cup,” Rouhani said.
“You will have to do your best in the competition and I believe that you can shine at the World Cup, However Team Melli have been drawn in a difficult group,” the President added.
Also present at the ceremony were Minister of Sports and Youth Affairs Masoud Soltanifar, Football Federation Islamic Republic of Iran President Mehdi Taj, as well as players and coaching staff.
Meanwhile, striker Kaveh Rezaei was omitted from Iran's final squad for the FIFA World Cup.
Rezaei, 26, enjoyed a good season with Belgium's Charleroi, scoring 16 goals in 39 games.
But Iran have strong choices up front, including Alireza Jahanbakhsh, who became the first Iranian to top the scoring table of a major European league with 21 goals for Dutch side AZ Alkmaar.
Iran squad
Goalkeepers: Alireza Beiranvand (Persepolis), Rashid Mazaheri (Zob Ahan), Amir Abedzadeh (Maritimo/POR);
Defenders: Ali Gholizadeh (Saipa), Majid Hosseini (Esteghlal), Milad Mohammadi (Akhmat Grozny/RUS), Mohammad Khanzadeh (Padideh), Morteza Pouraliganji (Alsaad/QAT), Pejman Montazeri (Esteghlal), Ramin Rezaeian (Ostende/BEL), Roozbeh Cheshmi (Esteghlal);
Midfielders: Ehsan Haji Safi (Olympiacos/GRE), Karim Ansarifard (Olympiacos/GRE), Masoud Shojaei (AEK Athens/GRE), Mahdi Torabi (Saipa), Omid Ebrahimi (Esteghlal), Saeid Ezatolahi (Amkar Perm/RUS);
Forwards: Alireza Jahanbakhsh (AZ Alkmaar/NED), Ashkan Dejageh (Nottingham Forest/ENG), Mahdi Taremi (Al-Gharafa/QAT), Reza Ghoochannejhad (Heerenveen/NED), Saman Ghoddos (Ostersunds/SWE), Sardar Azmoun (Rubin Kazan/RUS), Vahid Amiri (Persepolis).